URL: http://eprints.ucm.es/14832Colección: Archivo institucional e-prints complutense Autor: Alcolea, Sonia; Anton, Rosa; Bru, Isabel; Brú Espino, Antonio; Camacho, Mercedes; Porres, Amelia; Remacha, Angel; Soler, Marta; Souto Andres, Joan Carles; Vila, Luis CDU: 616 Departamento: Fac. de CC. Matemáticas - Depto. de Matemática Aplicada Editorial: HINDAWI PUBLISHING CORPORATION estatus: Publicado formato: Electrónico Identificador OAI: oai:www.ucm.es:14832 ISSN: 0962-9351 materia2: Análisis matemático Materia: Matemáticas Palabras clave: Acute inflammatory response; granulocyte-mediated cytotoxicity; colony-stimulating factor; human-melanoma cells; polymorphonuclear leukocytes; immune-response; nonoxidative mechanisms; endothelial-cells; cancer patients; gro-alpha; cell biology; Immunology Resumen: There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines-colorectal adenocarcinoma (HT-29) and pharyngeal squamous-cell carcinoma (FaDu) cells-to act as inflammatory foci. PMNs were cytotoxic to both lines, the adhesion of the PMNs to the tumour cells being important in this effect. The tumour cells released appreciable amounts of IL-8 and GRO alpha, and induced the transmigration of PMN through human microvascular-EC monolayers. Conditioning media associated with both lines induced the adhesion of PMN and the surface expression of ICAM-1 in microvascular-EC. In addition, FaDu-conditioning-medium strongly induced the production of proinflammatory cytokines by microvascular-EC. These results support the idea that tumour cells might normally induce a potent acute inflammatory response, leading to their own destruction. revisado: TRUE Tipo: Artículo Título: Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment Título de publicación: Mediators of Inflammation
Colección: Archivo institucional e-prints complutense